Fig. 3
Expression of known functional genes involved in the biopathways of tanshinones. Isoprenoid pathway: MEP pathway: the 2-C-methyl-d-erythritol 4-phosphate pathway; MVA pathway: the mevalonate pathway. Intermediate metabolites in the pathway: Acetoacetyl-CoA: Acetoacetyl coenzyme A; Acetyl-CoA: Acetyl coenzyme A; CDP-ME: 4-(Cytidine 5′-diphospho)-2-C-methyl-d-erythritol; DMAPP: Dimethylallyl pyrophosphate; DXP: 1-Deoxy-d-xylulose 5-phosphate; Ent-CPP: Ent-copalyl diphosphate; FPP: Farnesyl diphosphate; G3P: Glyceraldehyde 3-phosphate; GGPP: Geranylgeranyl diphosphate; GPP: Geranyl diphosphate; H-ME-B-PP: 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate; HMG-CoA: 3-Hydroxy-3-methylglutaryl CoA; IPP: Isopentenyl pyrophosphate; ME-cPP: 2-C-Methyl-d-erythritol 2,4-cyclodiphosphate; MEP: 2-C-Methyl-d-erythritol 4-phosphate; Mevalonate-P: Mevalonate-5-phosphate; Mevalonate-PP: Mevalonate-5-diphosphomevalonate; MVA: Mevalonate; PP-CDP-ME: 2-Phospho-4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol. Enzymes involving in the pathway: AACT: Acetoacetyl-CoA transferase; CMK: 4-(Cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase; CMS: 4-(Cytidine 5′-diphospho)-2-C-methyl-d-erythritol synthase; DXR: 1-deoxy-d-xylulose-5-phosphate reductoisomerase; DXS: 1-deoxy-d-xylulose-5-phosphate synthase; FPS: FPP synthase; GGPS: GGPP synthase; GPS: GPP synthase; HDR: 4-hydroxy-3-methyl-but-2-enyl diphosphate reductase; HDS: 4-Hydroxy-3-methylbut-2-enyl diphosphate synthase; HMGR: 3-Hydroxy-3-methylglutaryl CoA reductase; HMGS: Hydroxymethylglutaryl-CoA synthase; IDI: Isopentenyl-diphosphate delta-isomerase; MCS: 2-C-Methyl-d-erythritol 2,4-cyclodiphosphate synthase; MVK: Mevalonate kinase; PMD: Diphosphomevalonate decarboxylase; PMK: Phosphomevalonate kinase; SmCPS: Salvia miltiorrhiza copalyl diphosphate synthase; SmKSL: Salvia miltiorrhiza kaurene synthase-like