Table 2 Rat primer sequences of target genes
From: Topical application of Chinese herbal medicine DAEP relieves the osteoarthritic knee pain in rats
Primer | Forward/Reverse | Sequence (5′ to 3′) |
|---|---|---|
IL-6 | Forward | ATCTGCCCTTCAGGAACAGC |
Reverse | AGCCTCCGACTTGTGAAGTG | |
TNF-α | Forward | CAGCCGATTTGCCATTTCATAC |
Reverse | GGCTCTGAGGAGTAGACGATAA | |
iNOS | Forward | CTCAGGCTTGGGTCTTGTTAG |
Reverse | TGTTGTTGGGCTGGGAATAG | |
COX-2 | Forward | TCTCCAACCTCTCCTACTACAC |
Reverse | CTCCACCGATGACCTGATATTT | |
MMP-3 | Forward | GGACCAGGGATTAATGGAGATG |
Reverse | CAGGGTCCAGAGAGTTAGATTTG | |
GAPDH | Forward | CAACGACCCCTTCATTGACC |
Reverse | CGCCAGTAGACTCCACGACAT |