Fig. 2

EOFAZ inhibited retinal Müller gliosis in diabetic rats. After Immunohistochemical staining to GFAP and VEGF, the morphological images were taken under a microscope (a, b). Scale bar, 25 μm. ONL: Outer nuclear layer, INL inner nuclear layer, GCL ganglionic cell layer, OPL outer plexiform layer. Detection of VEGF level in serum (c), n ≥ 6. The expression of GFAP and VEGF in retina was detected by Western blot (d, e), and quantitative analysis via densitometry and was expressed as relative to β-actin. *P < 0.05, **P < 0.01 vs. non-diabetic rats,^#P < 0.05, ^##P < 0.01 vs. diabetic rats