Fig. 4

EOFAZ inhibited PPAR-γ downregulation and the high level of p-CREB and p- CaMKII in vivo and in vitro. Western blot analysis was used to analyze PPAR-γ, p-CREB (ser133), p-CaMK II (ser-286), total CREB and total CaMK II expression in rats (a, b). Western blots analysis was used to detect the expression of PPAR-γ, p-CREB (ser133), and p-CaMK II (ser-286), total CREB and total CaMKII levels in RMCs (c, d), mRNA level of PPAR-γ in RMCs detected by qRT-PCR (e). Data are expressed as mean ± SEM. n = 3. **P < 0.01 vs. non-diabetic rats or control group of RMCs, ^#P < 0.05, ^##P < 0.01 vs. Diabetic rats or HG group