Fig. 2

CPT inhibited TNF-α and IL-6 release as well as calcium influx in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. a, b The cells were pretreated with CPT (0, 2, 4, 8 μM) for 1 h before being stimulated with LPS for another 24 h. The pro-inflammatory cytokines, TNF-α and IL-6, were determined by ELISA. n = 3. c, d The intracellular calcium levels were measured by flow cytometry with Fluo-3AM (1 μM). n = 3. *p < 0.05, ***p < 0.001 compared with LPS alone group