Fig. 4

Inhibitory effects of CPT on lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-κB)/p65 activation and nuclear translocation in RAW264.7 cells. a, b The cells were pretreated with CPT (0, 2, 4, 8 μM) for 1 h before exposure to LPS (1 μg/mL) for 8 h. The protein expression of p65 was determined by immunoblotting. n = 3. c The cells were pretreated with CPT (0, 2, 4, 8 μM) for 1 h before exposure to LPS (1 μg/mL) for 2 h. The nuclear translocation of NF-κB/p65 was detected by immunofluorescence. n = 3. **p < 0.01, ***p < 0.001 compared with LPS alone group. Scale bar: 5 μm