Fig. 6

CPT exerted anti-inflammatory effects via toll-like receptor-4 (TLR4)/myeloid differentiation factor 88 (MyD88) pathway in RAW264.7 cells. The cells were pretreated with CPT (0, 2, 4, 8 μM) for 1 h before exposure to lipopolysaccharide (LPS; 1 μg/mL) for 8 h. The protein expressions of a, b TLR4, a, c MD-2, a, d MyD88, and a, e transforming growth factor beta-activated kinae 1 (Tak1) were determined by immunoblotting. n = 3. **p < 0.01, ***p < 0.001 compared with LPS alone group