Fig. 2
From: Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis
The effects of SCU on the proliferation and apoptosis of HCT-116 and RKO cells. a Immunofluorescent staining of TUNEL in Control, IC20 and IC50 groups. Apoptotic cells are stained by red color, and the nucleus is stained by blue. b The apoptosis rate (TUNEL/DAPI) comparison among these three groups. The flow cytometry detection of c HCT-116 cells and d RKO cells in the indicated groups. The cell count variation of e HCT-116 cells and f RKO cells in Control, IC20 and IC50 groups. g The variation of Caspase3/7 activity in control, IC20 and IC50 groups. IC20: 20% inhibition concentration; IC50, 50% inhibition concentration; SCU, Scutellarin; TUNEL, Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling; DAPI, 4′,6-diamidino-2-phenylindole. All data are shown as mean ± SD, n = 5. *p < 0.05. Scale bar = 100 μm