Fig. 4

OMs inhibited IAPP induced mitochondrial dysfunction in RINm5F cells. The Islet β cells were seeded in 6-well plates and treated with OMs at 50 μM for 48 h, and then induced with IAPP (250 nM) for 24 h. After treatment, cells were presented for oxygen consumption in that equal volumes of cells were separated into aliquots in wells of a 96-well BD Oxygen Biosensor plate. Fluorescence in each well was recorded over time. a Representative oxygen consumption curves. b Quantitative changes in the respiratory rate of the islet β cells calculated by determining the kinetic measurements. Or the mitochondrial of RINm5F cells were isolated for detection of complex activities. c Mitochondrial complex I activity assay. d Mitochondrial complex II activity assay. e Mitochondrial complex III activity assay. Values are mean ± S.E.M of the results from at least three independent experiments. ^##P < 0.01 versus control, *P < 0.05 versus the IAPP model