Fig. 5

OM3.0 suppressed JNK activation and cell apoptosis in IAPP induced islet β cells. RINm5F cells in 6-well plates were treated with OMs at 50 μM for 48 h, and then induced with IAPP (250 nM) for 24 h. a Inhibition of JNK activation by OM3.0 treatment. Cell lysates were subjected to western blot analysis of phosporylated JNK. α-tubulin was used as an internal control. b Effect of OM3.0 on protein expression of Bcl-2 and Bax. Cell lysates were subjected to westrern blot analysis of Bcl-2 and Bax, α-tubulin was used as an internal control. c OM3.0 prevented IAPP induced changes of Cyto-c and AIF release from mitochondria into cytosol. The treated cells were separated into cytosolic and mitochondrial fractions. Cell lysates were subjected to western blot analysis. Changes in levels of protein expression (shown as ratios) were calculated based on levels in corresponding untreated cells, which were set at unity. Data represent similar results from three independent experiments