Table 2 Protocol of experimental design
Groups | n | Tested substance(s) | Description |
|---|---|---|---|
A. Acute (6Â h) and subchronic (8Â days) effect of Fumaria officinalis (FO) on blood glucose levels | |||
 I | 7 | Control | Normal mice: Vehicle [sterile cold saline (0.9%)], PO |
 II | 7 | Diabetic Control | Diabetic mice: Vehicle, PO |
 III | 7 | GB | Diabetic mice: GB 5 mg/kg, PO |
 IV | 7 | FO | Diabetic mice: FO 50 mg/kg, PO |
 V | 7 | FO | Diabetic mice: FO 100 mg/kg, PO |
 VI | 7 | FO | Diabetic mice: FO 200 mg/kg, PO |
 VII | 7 | ARF | Diabetic mice: ARF 15 mg/kg, PO |
 VIII | 7 | ARF | Diabetic mice: ARF 30 mg/kg, PO |
 IX | 7 | ARF | Diabetic mice: ARF 60 mg/kg, PO |
 X | 7 | Placebo I | Diabetic mice: Placebo I (Nio 3), PO |
 XI | 7 | Nio 1 | Diabetic mice: ARF 60 mg/kg in Nio 1, PO |
 XII | 7 | Nio 2 | Diabetic mice: ARF 60 mg/kg in Nio 2, PO |
 XIII | 7 | Nio 3 | Diabetic mice: ARF 60 mg/kg in Nio 3, PO |
 XIV | 7 | Placebo II | Diabetic mice: Placebo II (Nio 6), PO |
 XV | 7 | Nio 4 | Diabetic mice: ARF 60 mg/kg in Nio 4, PO |
 XVI | 7 | Nio 5 | Diabetic mice: ARF 60 mg/kg in Nio 5, PO |
 XVII | 7 | Nio 6 | Diabetic mice: ARF 60 mg/kg in Nio 6, PO |
 XVIII | 7 | Sty | Diabetic mice: Sty 7.5 mg/kg, PO |
 XIX | 7 | Sty | Diabetic mice: Sty 15 mg/kg, PO |
 XX | 7 | Sty | Diabetic mice: Sty 30 mg/kg, PO |
 XXI | 7 | San | Diabetic mice: San 7.5 mg/kg, PO |
 XXII | 7 | San | Diabetic mice: San 15 mg/kg, PO |
 XXIII | 7 | San | Diabetic mice: San 30 mg/kg, PO |
B. For longer time (0, 2, 4, 6 and 8Â weeks) effects on hot plate and tail withdrawal latencies, and von Frey paw withdrawal thresholds | |||
 XXIII | 7 | Control | Normal mice: Vehicle [sterile cold saline (0.9%)], PO |
 XXIV | 7 | Vehicle Control | Diabetic mice: Vehicle, PO |
 XXV | 7 | GB | Diabetic mice: GB 5 mg/kg, PO |
 XXVI | 7 | FO | Diabetic mice: FO 50 mg/kg, PO |
 XXVII | 7 | FO | Diabetic mice: FO 100 mg/kg, PO |
 XXVIII | 7 | FO | Diabetic mice: FO 200 mg/kg, PO |
 XXIX | 7 | ARF | Diabetic mice: ARF 15 mg/kg, PO |
 XXX | 7 | ARF | Diabetic mice: ARF 30 mg/kg, PO |
 XXXI | 7 | ARF | Diabetic mice: ARF 60 mg/kg, PO |
 XXXII | 7 | Placebo I | Diabetic mice: Placebo I (Nio 3), PO |
 XXXIII | 7 | Nio 1 | Diabetic mice: ARF 60 mg/kg in Nio 1, PO |
 XXXIV | 7 | Nio 2 | Diabetic mice: ARF 60 mg/kg in Nio 2, PO |
 XXXV | 7 | Nio 3 | Diabetic mice: ARF 60 mg/kg in Nio 3, PO |
 XXXVI | 7 | Placebo II | Diabetic mice: Placebo II (Nio 6), PO |
 XXXVII | 7 | Nio 4 | Diabetic mice: ARF 60 mg/kg in Nio 4, PO |
 XXXVIII | 7 | Nio 5 | Diabetic mice: ARF 60 mg/kg in Nio 5, PO |
 XXXIX | 7 | Nio 6 | Diabetic mice: ARF 60 mg/kg in Nio 6, PO |
 XXXX | 7 | Sty | Diabetic mice: Sty 7.5 mg/kg, PO |
 XXXXI | 7 | Sty | Diabetic mice: Sty 15 mg/kg, PO |
 XXXXII | 7 | Sty | Diabetic mice: Sty 30 mg/kg, PO |
 XXXXIII | 7 | San | Diabetic mice: San 7.5 mg/kg, PO |
 XXXXIV | 7 | San | Diabetic mice: San 15 mg/kg, PO |
 XXXXXV | 7 | San | Diabetic mice: San 30 mg/kg, PO |