Fig. 4

Uvaol inhibits mRNA expression and production of inflammatory mediators of LPS-stimulated RAW264.7 cells. a Cytotoxicity of uvaol on RAW264.7 cells. Uvaol was incubated with RAW264.7 cells for 24 h, and cell viability was detected by CCK8 kit. Data are expressed as mean ± SEM, n = 6. b NO release in LPS- stimulated RAW264.7 cells. Data are expressed as mean ± SEM, n = 4. c The microscopic morphological changes of LPS-induced RAW264.7 cells (magnification 20 ×). d mRNA expression of TNF-α, MCP-1, IL-6 and IL-1β of LPS-stimulated RAW264.7 cells. e TNF-α, IL-6 and IL-1β protein concentration in supernatants of LPS-stimulated RAW264.7 cells. f COX-2 and iNOS expressions of LPS-stimulated RAW264.7 cells. Data are expressed as mean ± SEM, n = 3. The images shown are representatives of three independent experiments.^###P < 0.001 and^##P < 0.01, compared with control group; ***P < 0.001, **P < 0.01 and *P < 0.05, compared with LPS- stimulated group