Fig. 5

Uvaol inhibits STAT3 and ERK activation in colon tissues of DSS-treated mice and LPS-stimulated RAW264.7 cells. a STAT3, p-STAT3, ERK and p-ERK expressions in colon tissues of DSS-treated mice. Colitis was induced in all groups except the control group. Uvaol and CsA were administered to mice from day 8 to day 14. On day 14, the mice were sacrificed, and protein expression of STAT3, p-STAT3, ERK and p-ERK in colon homogenates were determined by western blotting. Data are expressed as mean ± SEM, n = 6. b STAT3, p-STAT3, ERK and p-ERK expression in LPS-stimulated RAW264.7 cells. RAW264.7 cells were treated with 1 μg/mL of LPS in the absence or presence of designated concentrations of uvaol for 3 h, Data are expressed as mean ± SEM, n = 3. The images shown are representatives of three independent experiments.^###P < 0.001 and^##P < 0.01, compared with control group; ***P < 0.001, **P < 0.01 and *P < 0.05, compared with DSS or LPS- stimulated group