Fig. 5

Masson Trichrome staining of tissues with different treatments on days 7, 14 and 28, 20×. a CA on day 7, the epithelium did not migrate remarkably from the edge (blackbroken line circle); b TA1 on day 7, the epithelial hyperplasia has migrated less than 50% of the distance (blackbroken line circle); c TA2 on day 7, less than 50% of the epithelium has migrated above the granulation tissue (blackbroken line circle); d TA3 on day 7, less than 50% of the epithelium has migrated above the granulation tissue (blackbroken line circle); e CA on day 14, the epithelial hyperplasia has migrated above the granulation tissue. Only some collagen observed in the granulation tissue; f TA1 on day 14, the fibroblasts have secreted very limited amounts of collagen in the granulation tissue; g TA2 on day 14, similar progress as TA1 on day 14; h TA3 on day 14, similar progress as TA1 on day 14; i CA on day 28, the epithelium was bridged (black circle), only limited collagen labeled in the granulation tissue; j TA1 on day 28, the collagen has crossed the border and has extended to the center (white asterisk), note the epithelium has finished regenerating with epidermal ridge reconstruction; k TA2 on day 28, similar epithelial restoration as TA1 with less collagen in the granulation tissue (white asterisk); l TA3 on day 28, sporadic bridging has occurred (black circle) with less collagen deposition (white asterisk) than TA1