Fig. 4
Identification of hub genes through DESeq2. RA-FLSs were exposed to 3 mg/mL of WJT extract for 24 h and 48 h, and RNA was extracted for RNA-seq. Principal component analysis (PCA) of the mRNA expression among different groups (a). Volcano plot of the differentially expressed genes (DEGs) among different groups (b). Red DEGs: |log2(fold-change)| < 1; turquoise DEGs: |log2(fold-change)| > 1. Venn diagram of the DEGs among different groups, showing 184 common DEGs (6 upregulated and 178 downregulated) (c). Heat map of the 184 common DEGs among samples (d). Dotplot of the GO enrichment analysis (e) and the KEGG enrichment analysis (f) for the 184 DEGs. Protein-protein interaction (PPI) network of the DEGs in the significantly enriched pathways, and the node degrees in PPI relationship (d). n = 3 per group