Table 1 Primer sequences used for qRT-PCR
Gene | Primer | Sequence (5′→3′) |
|---|---|---|
BUB1 | Forward | AGCCCAGACAGTAACAGACTC |
Reverse | GTTGGCAACCTTATGTGTTTCAC | |
THOC1 | Forward | GAAAAATGAAGGTTGCCCAAGTT |
Reverse | TTGTCTCTGATTTACAGGCTTCC | |
SMC3 | Forward | AACATAATGTGATTGTGGGCAGA |
Reverse | TCCTTTTTGGCACCAATAACTCT | |
STAG2 | Forward | TCCTTCTGGTCCAAACCGAAT |
Reverse | ACCGACTGCATAGCACTCTTG | |
ORC4 | Forward | AGATTTTCTCACCGGCAGATACA |
Reverse | AGCAAGCATCAATAGCATGTGT | |
TTK | Forward | TCATGCCCATTTGGAAGAGTC |
Reverse | CCACTTGGTTTAGATCCAGGC | |
ATR | Forward | TCCCTTGAATACAGTGGCCTA |
Reverse | TCCTTGAAAGTACGGCAGTTC | |
BRCA1 | Forward | GAAACCGTGCCAAAAGACTTC |
Reverse | CCAAGGTTAGAGAGTTGGACAC | |
EIF3A | Forward | ACTCAGGATCGTACTGACAGATT |
Reverse | ATGGTACAGGCGCTCTACTCT | |
PNN | Forward | CTGGTCCTGGTGGAGGTAGA |
Reverse | TGTGCGCTCTTTGGAGGTAG | |
GAPDH | Forward | GGAGCGAGATCCCTCCAAAAT |
Reverse | GGCTGTTGTCATACTTCTCATGG |