Fig. 2

Positive correlation between the expression of IFI16 and caspase-1 in laryngeal carcinoma. a The protein expression of IFI16, caspase-1, IL-1β in 3 pairs of laryngeal carcinoma and adjacent normal tissues from 3 LSCC patients. N and T are used to represent adjacent and cancer tissues, respectively. β-actin served as the loading control. b Statistical analysis of IFI16 inflammasomes. Data were shown as mean ± SD (n = 3). ‘*’ represents that P < 0.05 vs. adjacent normal tissues. c IFI16 mRNA expression in different TNM stages in HNSCC patients (GEPIA). d The correlation between IFI16 mRNA expression and Caspase-1 in HNSCC patients in the GEPIA database. E-H. IFI16 and caspase-1 expression is analyzed via immunohistochemistry for 36 biopsy specimens of laryngeal carcinoma tissues. e IFI16 exhibited weak nuclear staining in 8 of the 36 (22.2%). f IFI16 exhibited strong nuclear staining in 28 of the 36 specimens (77.8%). g Caspase-1 exhibited weak cytoplasmic staining in 16 of the 36 specimens (44.4%). h Caspase-1 exhibited strong cytoplasmic staining in 20 of the 36 specimens (55.6%). Original magnification, ×400. Scale bars, 25 μm, Black arrow