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Fig. 5 | Chinese Medicine

Fig. 5

From: RalB degradation by dihydroartemisinin induces autophagy and IFI16/caspase-1 inflammasome depression in the human laryngeal squamous cell carcinoma

Fig. 5

DHA suppressed the activation of IFI16 inflammasome and promoted autophagy in vitro. a Western blot was used to detect the expression of IFI16‐mediated inflammasome and LC3B in Hep-2 and Cal-27 cells. Cells were treated as described above. GAPDH was used as the loading control. b DHA-induced autophagosomes were detected in Hep-2 cells by immunofluorscent staining of LC3B (green) (×1000). Hep-2 Cells were treated with DHA (20.2 μM) and 0.1% DMSO (NC), respectively for 24 h. F-actin (red) was stained with phalloidine (red). Nuclei were counter-stained with DAPI (blue). The upper and bottom panels were respectively ×400 and ×1000. The bar chart in the lower right corner was shown as the result of statistical analysis of the autophagosome number. A total of 200 cells were counted for each group. Data were shown as mean ± SD (n = 3). *P < 0.05 vs. NC group. c The formation of autophagic vacuoles by DHA was observed under transmission electron microscope. The autophagic vacuole was indicated by blue asterisks. A total of 20 cells were counted for each group. d The expression of IFI16-mediated inflammasome, LC3B and p62/SQSTM1 was detected by Western blot. Hep-2 cells were exposed to 20.5 μM DHA and/or 50 μM Chloroquine (CQ; Sigma, USA) for 24 h. Data were expressed as mean ± SD (n = 3). *P < 0.05 vs. NC group. #P < 0.05 vs. CQ group

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