Fig. 6

Connecting the inflammasome and autophagy pathways. a Western blot was used to detect the expression of IFI16 and RalB in Hep-2, Cal-27 and HeLa cells. GAPDH was used as the loading control. Hep-2, Cal-27 and HeLa cells were treated with DHA (20.2 μM, 24.5 μM, 31 μM), and 0.1% DMSO (NC) respectively for 24 h. Rapamycin (0.1 μM) acted as the autophagy activator, and 3-MA (1 mM) acted as the autophagy inhibitor. b Statistical analysis of IFI16, RalB and USP33. The cells were treated as described above. Data were shown as mean ± SD (n = 3). ‘*’ represents that P < 0.05 vs. NC