Table 1 The sequence of primers for real-time PCR
Gene name | Primer sequence (5′-3′) |
|---|---|
TGF-β1 | Sense: CCTGGAAAGGGCTCAACAC |
Antisense: CAGTTCTTCTCTGTGGAGCTGA | |
Smad2 | Sense: CAGGACGATTAGATGAGCTTGA |
Antisense: CCCCAAATTTCAGAGCAAGT | |
Smad3 | Sense: CCTGCCACTGTCTGCAAG |
Antisense: GCAGCAAATTCCTGGTTGTT | |
Smad7 | Sense: ACCCCCATCACCTTAGTCG |
Antisense: AAATCCATCGGGTATCTGGA | |
CD105 | Sense: TGGGAGGCTAGGACTCAAGA |
Antisense: TTGTGCTAGTCAAGGTGAGGAG | |
GAPDH | Sense: TGTTGCCATCAACGACCCCTT |
Antisense: CTCCACGACATACTCAGCA |