An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma

Table 1 The information of compound ionization

Analyte	Retention time (min)	MRM transition (m/z)		Dwell time (msec)	Q₁ pre bias (V)	Collision energy (V)	Q₃ pre bias (V)
Analyte	Retention time (min)	Precursor ion	Product ion	Dwell time (msec)	Q₁ pre bias (V)	Collision energy (V)	Q₃ pre bias (V)
1	2.022	347.05	185.10	22	− 21	− 13	− 18
2	2.079	154.20	94.00	22	− 25	− 10	− 26
3	2.184	254.15	206.05	22	− 15	− 10	− 25
4	2.464	254.20	206.05	22	− 15	− 10	− 21
5	2.539	481.2	23.05	22	− 26	− 45	− 29
6	3.206	195.05	89.00	22	− 25	− 33	− 16
7	3.571	431.10	268.05	22	16	30	28
8	3.835	137.10	93.05	22	10	16	29
9	3.837	338.20	323.10	22	− 25	− 24	− 15
10	3.979	431.10	269.10	22	− 24	− 22	− 28
11	4.324	352.20	336.10	22	− 19	− 31	− 16
12	4.355	395.25	331.10	22	28	12	22
13	4.461	336.15	320.15	22	− 24	− 31	− 15
14	5.701	273.10	153.00	22	− 30	− 22	− 15
15	7.354	333.2	292.05	31	− 19	− 10	− 30
16	8.928	315.10	300.05	46	− 26	− 22	− 20
17	9.391	319.25	301.15	46	− 30	− 13	− 20
18	9.670	191.10	77.05	46	− 13	− 41	− 29
19	9.715	299.10	283.00	46	− 30	− 32	− 19
I.S.	9.186	325.15	149.00	46	− 30	− 35	− 20

Dwell time: residence time during an acquisition point; Q1 pre bias: voltage promotes the ionization of the precursor ion; Q3 pre bias: voltage promotes the ionization of the product ion

ISSN: 1749-8546