An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma

Table 2 The linear regression equation, linear range, LLOQ and LLOD of 19 compounds in FKQJF

Analyte	Calibration curve	r²	Linear range (ng/mL)	LLOD (ng/mL)	LLOQ (ng/mL)
1	y = 0.0021x + 0.015	0.9993	4.88–625	0.73	2.12
2	y = 0.0906x + 1.9797	0.9923	4.88–625	0.85	2.08
3	y = 0.0075x + 0.1078	0.9941	4.88–625	0.64	1.97
4	y = 0.0053x + 0.062	0.9964	4.88–625	0.78	1.96
5	y = 0.0012x + 0.018	0.9956	4.88–625	1.25	2.58
6	y = 0.0009x + 0.0019	0.9991	5.81–743.75	0.68	2.45
7	y =0.0035x + 0.0124	0.9910	5.13–656.25	1.24	2.32
8	y = 0.0003x + 0.0416	0.9940	4.88–625	1.12	2.53
9	y = 0.0816x + 0.6041	0.9936	1.56–200	0.23	0.76
10	y = 0.0108x + 0.2454	0.9926	4.88–625	0.76	2.36
11	y = 0.1335x + 0.6769	0.9957	2.37–303.13	0.81	1.58
12	y = 0.0007x + 0.015	0.9932	6.01–768.75	1.02	2.98
13	y = 0.1113x + 0.6035	0.9980	2.73–350	0.88	1.75
14	y = 0.0052x − 0.0095	0.9994	4.88–625	0.59	2.06
15	y = 0.076x + 0.8723	0.9954	4.88–312.5	0.92	2.43
16	y = 0.006x + 0.0716	0.9925	4.98–637.5	0.88	2.43
17	y = 0.0058x + 0.0282	0.9984	6.01–768.75	1.04	3.32
18	y = 0.0001x + 0.0209	0.9964	4.05–518.75	0.93	2.33
19	y = 0.0113x + 0.0195	0.9983	3.52–450	0.34	1.05

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