An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma

Table 3 The precision and accuracy of 19 compounds in FKQJF

Analyte	Spiked (ng/mL)	Intra-day		Inter-day
Analyte	Spiked (ng/mL)	Precision (RSD %)	Accuracy (%)	Precision (RSD %)	Accuracy (%)
1	500	14.94	93.93	12.08	82.52
	250	6.65	108.26	6.22	91.24
	50	13.39	113.94	8.75	85.02
2	500	12.48	112.91	13.95	80.94
	250	8.48	80.88	13.18	93.34
	50	6.27	96.72	5.90	90.32
3	500	10.61	108.40	6.47	89.09
	250	14.24	100.18	13.93	99.35
	50	8.71	82.38	4.33	97.38
4	500	4.84	112.84	8.43	97.25
	250	5.27	100.10	6.41	119.43
	50	9.46	87.00	8.11	88.31
5	600	7.81	108.38	8.47	112.68
	300	14.76	86.11	9.76	105.33
	60	11.11	110.49	14.19	103.58
6	600	10.81	114.84	9.43	94.40
	300	7.90	83.02	13.57	106.38
	60	10.82	108.04	8.61	90.00
7	500	13.68	92.90	4.31	98.35
	250	12.95	81.97	10.88	98.16
	50	5.48	96.41	5.00	101.20
8	500	8.68	92.52	14.81	104.02
	250	8.61	85.92	5.69	106.35
	50	10.67	117.97	8.19	99.10
9	150	9.92	109.50	6.29	111.14
	75	13.28	89.18	6.03	84.78
	25	10.13	87.51	5.53	80.03
10	500	4.92	103.61	13.94	107.44
	250	10.71	91.96	9.03	98.62
	50	13.94	102.51	5.47	81.71
11	200	8.87	86.93	11.32	89.26
	100	6.63	118.80	7.25	93.71
	5	6.64	107.27	4.85	96.59
12	600	7.59	113.80	12.96	105.22
	300	7.40	103.81	12.45	110.32
	60	7.82	118.22	11.99	109.08
13	200	9.81	89.49	7.92	89.26
	100	4.34	108.30	4.10	99.07
	5	8.16	92.97	13.95	108.55
14	500	5.45	86.37	6.65	93.67
	250	10.77	119.76	14.21	83.17
	50	4.49	83.50	9.89	119.30
15	600	13.10	112.68	8.06	110.97
	300	6.90	91.02	10.91	88.92
	60	13.93	103.81	9.02	92.39
16	500	5.70	85.56	13.38	87.62
	250	11.61	89.73	14.78	100.02
	50	9.72	118.83	10.08	96.61
17	600	8.29	109.97	7.57	95.28
	300	4.62	102.48	4.25	93.29
	60	14.85	90.07	4.57	99.00
18	400	4.68	100.57	12.66	109.09
	200	10.44	115.16	10.62	87.68
	20	12.41	116.16	13.41	87.29
19	300	4.83	115.96	13.09	91.69
	150	7.15	106.77	9.09	115.94
	10	6.41	80.33	11.85	89.01