Fig. 4

Effects of HWG, RA and CDDP synergistically arrest the cell cycle at G2/M phase and apoptosis in RCC 786-O cells. 786-O cells were treated with the vehicle control, CDDP (5 μM) alone, HWG (50 ~ 200 μg/mL), RA alone, or HWG combined with CDDP (a), and RA (25 ~ 100 μM) combined with CDDP (b) incubated for 48 h. Cell cycle distribution based on DNA content was analyzed through flow cytometry. Different cell phases were plotted as the percentage of total events. Data are presented as the mean ± SD of at least three independent experiments. ^∗P  < 0.05, ^∗∗P  < 0.01, and ^∗∗∗P  < 0.001 compared with CDDP