Exploring protective effect of Glycine tabacina aqueous extract against nephrotic syndrome by network pharmacology and experimental verification

Table 1 Identification of the chemical components in GATE by UHPLC-DAD-MS/MS analysis (negative ion mode)

Peak	Retention time (min)	Experimental [M−H]⁻ (m/z)	Theoretical [M−H]⁻ (m/z)	Delta (ppm)	Main MS² fragments	UV max. (nm)	Molecular formula	Proposed compound
1	15.26	431.09823	431.09837	− 0.325	369.18481, 329.13257, 269.13925, 203.05310	260	C₂₁H₂₀O₁₀	Genistin
2	19.31	415.10405	415.10346	1.421	253.10510	255	C₂₁H₂₀O₉	Daidzin
3	20.19	563.14050	563.14063	− 0.228	503.28558, 473.20331, 443.26880, 383.15466, 353.13293	273, 334	C₂₆H₂₈O₁₄	Isoschaftoside
4	29.46	253.05037	253.05063	− 1.036	224.17744, 209.02998, 197.07283, 134.95865	250, 303	C₁₅H₁₀O₄	Daidzein
5	30.30	283.06104	283.06120	− 0.554	268.09027	255	C₁₆H₁₂O₅	Glycitein
6	32.58	269.04538	269.04555	− 0.619	241.05565, 225.15002, 181.10497	261	C₁₅H₁₀O₅	Genistein

ISSN: 1749-8546