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Fig. 5 | Chinese Medicine

Fig. 5

From: Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model

Fig. 5

EF&LLF regulated bone turnover rate. a, b Serum bALP and TRACP were measured by ELISA. c bALP/TRACP in serum was calculated. d, e Activity of ALP or TRACP in tibia was measured using ALP or TRACP stain kit. f ALP/TRACP in tibia was calculated. g Representative photographs of ALP and TRACP staining were showed (× 200). Arrow (black): ALP-stained positive cell (osteoblast). Arrow (red): TRACP-stained positive cell (osteoclast). Mean ± SEM, n = 6 or 8. **P < 0.01 vs. 6 m; â–³P < 0.05 and △△P < 0.01 vs. 17 m; ▲▲P < 0.01 vs. EF&LLF

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