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Fig. 7 | Chinese Medicine

Fig. 7

From: Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model

Fig. 7

EF&FLL up-regulated the expression of OPG, RANKL, ATP6v0d2 and M-CSF. a Bone OPG mRNA was by qPCR analysis with β-actin as an internal control. b Bone OPG protein was measured by WB. c Serum OPG was by ELISA. d Bone RANKL mRNA was by qPCR. e Bone RANKL protein was measured by WB. f Serum RANKL was tested by ELISA. g OPG/RNAKL in bone mRNA, bone protein and serum were calculated. h mRNA and protein of ATP6v0d2 was measured by qPCR and WB, respectively. i M-CSF mRNA was tested by qPCR. j M-CSF protein was measured by WB. k Representative WB photographs of OPG, RANKL, ATP6v0d2 and M-CSF were viewed, and β-actin or β-Tubulin was used for normalization. Mean ± SEM, n = 6. *P < 0.05 and **P < 0.01 vs. 6 m; P < 0.05, P < 0.01 vs. 17 m; P < 0.05, ▲▲P < 0.01 vs. EF&LLF

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