Fig. 7

EF&FLL up-regulated the expression of OPG, RANKL, ATP6v0d2 and M-CSF. a Bone OPG mRNA was by qPCR analysis with β-actin as an internal control. b Bone OPG protein was measured by WB. c Serum OPG was by ELISA. d Bone RANKL mRNA was by qPCR. e Bone RANKL protein was measured by WB. f Serum RANKL was tested by ELISA. g OPG/RNAKL in bone mRNA, bone protein and serum were calculated. h mRNA and protein of ATP6v0d2 was measured by qPCR and WB, respectively. i M-CSF mRNA was tested by qPCR. j M-CSF protein was measured by WB. k Representative WB photographs of OPG, RANKL, ATP6v0d2 and M-CSF were viewed, and β-actin or β-Tubulin was used for normalization. Mean ± SEM, n = 6. *P < 0.05 and **P < 0.01 vs. 6 m; ^△P < 0.05, ^△△P < 0.01 vs. 17 m; ^▲P < 0.05, ^▲▲P < 0.01 vs. EF&LLF