Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model

Table 1 Primer used for qPCR

Primer	Upstream	Downstream	Product size
RANKL	GCAGCATCGCTCTGTTCCTGTA	GCATGAGTCAGGTAGTGCTTCTGTG	164 bp
M-CSF	GAATGACTGAACCTGCCTGCTGAA	AGGCCAGCTCAGTGCAAGAA	117 bp
Wnt5a	ACAGGCAGTGGCATGCAGA	CAGGCAGCTGTTGACCTAGGAA	134 bp
Atp6v0d2	CGAGGCATTCTACAAATTCTGCAA	TTCAGTGCCAAATGAGTTCAGAGTG	124 bp
OPG	CTCATCAGTTGGTGGGAATGAAGA	ACCTGGCAGCTTTGCACAATTA	107 bp
IGF-1	GCACTCTGCTTGCTCACCTTTA	TCCGAATGCTGGAGCCATA	148 bp
TGF-β1	CATTGCTGTCCCGTGCAGA	AGGTAACGCCAGGAATTGTTGCTA	103 bp
BMP2	ACCGTGCTCAGCTTCCATCAC	CTATTTCCCAAAGCTTCCTGCATTT	170 bp
β-actin	CACTTTCTACAATGAGCTGCG	CTGGATGGCTACGTACATGG	129 bp

RANKL nuclear factor-kappa B ligand, M-CSF macrophage colony-stimulating factor, Atp6v0d2 d2 isoform of vacuolar (H +) ATPase, OPG osteoprotegerin, IGF-1 insulin-like growth factor-1, TGF-β1 transforming growth factor-β1, BMP2 bone morphogenetic protein-2

ISSN: 1749-8546