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Fig. 3 | Chinese Medicine

Fig. 3

From: Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation

Fig. 3

SGE upregulated the level of CD25+CD4+FOXP3+ cells in spleen. The purified splenocytes (5 ~ 10 × 105 cells) were surface-stained with anti-rat CD4-FITC/CD25-PE for 30 min on ice in darkness, then permeabilized and stained with anti-mouse/rat/human Foxp3-Alexa Fluor® 647 for another 30 min. The acquisition of flow cytometry data and the analysis of a cell population of at least 1 × 104 splenocytes were performed on flow cytometer with the BD FACSDiva Software (FACSCanto, BD Biosciences). Representative images of flow cytometry for CD4+CD25+Foxp3+ regulatory T cells (a). Quantification of the number of CD4+CD25+Foxp3+ regulatory T cells (b). (##P < 0.01 vs. Ctrl group; **P < 0.01 vs. CIA group; n = 6)

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