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Table 1 Linearity and sensitivity of UPLC-MS/MS assay for standards in fermented DBT

From: Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Analyte Calibration curve R2a Linear range (μg/mL) LLOQ (μg/mL) LOD (μg/mL)
Cal-O-β-glud y = 0.1032x + 0.0397 0.9986 0.023–50 0.023 0.008
Ononin y = 0.2496x + 0.1498 0.9967 0.023–50 0.023 0.008
Calycosin y = 4.4401x + 4.0138 0.9909 0.023–50 0.023 0.008
Formononetin y = 3.8437x + 3.2295 0.9923 0.023–50 0.023 0.008
  1. aThe calibration curve was constructed by plotting the peak area versus the concentration of each analyte. Each calibration curve was derived from 6 data points, n = 3. R2, coefficient of determination
  2. LLOQ lower limit of quantification
  3. LOD limit of detection
  4. Cal-O-β-glud calycosin-7-O-β-D-glucoside