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Table 2 Intra-day and inter-day precision and accuracy for UPLC-MS/MS assay of standards in fermented DBT

From: Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Analyte Spiked (μg/mL) Intra-day (n = 6)a Inter-day (n = 18)b
Measured (μg/mL) Precision (RSD%) Accuracy (RE%) Measured (μg/mL) Precision (RSD%) Accuracy (RE%)
Cal-O-β-glu 0.07 0.07 4.52 2.77 0.07 4.09 4.24
1.84 1.91 0.84 4.16 1.98 2.30 7.93
16.60 16.30 2.04 − 1.97 16.70 1.74 − 0.14
Ononin 0.07 0.07 2.83 − 2.30 0.06 6.09 − 5.03
1.84 2.03 1.92 10.20 2.09 2.48 13.90
16.60 14.80 2.32 − 12.80 14.90 1.44 − 10.30
Calycosin 0.07 0.07 2.06 4.42 0.06 8.51 − 10.80
1.84 2.05 1.32 11.50 2.04 2.56 11.20
16.60 15.50 2.21 − 7.58 16.00 1.10 − 6.51
Formononetin 0.07 0.07 2.61 3.93 0.06 2.44 − 6.58
1.84 1.99 1.91 7.99 1.99 2.63 7.88
16.60 15.60 2.20 − 6.87 15.80 1.02 − 4.90
  1. aThe intra-day analysis refers to the sample examined for 6 replicates within one day
  2. bThe inter-day analysis refers to the sample examined in duplicates over 3 consecutive days
  3. Cal-O-β-glu calycosin-7-O-β-D-glucoside