Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Table 2 Intra-day and inter-day precision and accuracy for UPLC-MS/MS assay of standards in fermented DBT

Analyte	Spiked (μg/mL)	Intra-day (n = 6)^a			Inter-day (n = 18)^b
Analyte	Spiked (μg/mL)	Measured (μg/mL)	Precision (RSD%)	Accuracy (RE%)	Measured (μg/mL)	Precision (RSD%)	Accuracy (RE%)
Cal-O-β-glu	0.07	0.07	4.52	2.77	0.07	4.09	4.24
	1.84	1.91	0.84	4.16	1.98	2.30	7.93
	16.60	16.30	2.04	− 1.97	16.70	1.74	− 0.14
Ononin	0.07	0.07	2.83	− 2.30	0.06	6.09	− 5.03
	1.84	2.03	1.92	10.20	2.09	2.48	13.90
	16.60	14.80	2.32	− 12.80	14.90	1.44	− 10.30
Calycosin	0.07	0.07	2.06	4.42	0.06	8.51	− 10.80
	1.84	2.05	1.32	11.50	2.04	2.56	11.20
	16.60	15.50	2.21	− 7.58	16.00	1.10	− 6.51
Formononetin	0.07	0.07	2.61	3.93	0.06	2.44	− 6.58
	1.84	1.99	1.91	7.99	1.99	2.63	7.88
	16.60	15.60	2.20	− 6.87	15.80	1.02	− 4.90

^aThe intra-day analysis refers to the sample examined for 6 replicates within one day
^bThe inter-day analysis refers to the sample examined in duplicates over 3 consecutive days
Cal-O-β-glu calycosin-7-O-β-D-glucoside

ISSN: 1749-8546