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Table 3 Extraction efficiency, matrix effect and stability for UPLC-MS/MS assay of flavonoids in fermented DBT

From: Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Analyte Spiked (μg/mL) Extraction efficiencya (n = 6) Matrix effectsb (n = 6) Autosampler stabilityc (n = 6)
Measured (%) RSD (%) Measured (%) RSD (%) Accuracy (RE%) RSD (%)
Cal-O-β-glu 0.07 101.20 2.49 114.30 8.18 4.29 11.50
1.84 95.00 2.45 104.10 1.82 8.57 2.51
16.60 99.20 1.82 110.20 1.13 1.33 1.48
Ononin 0.07 102.10 6.90 99.80 3.89 2.82 5.81
1.84 93.30 2.95 97.20 2.61 14.80 1.95
16.60 100.00 3.42 81.50 0.91 -7.68 2.41
Calycosin 0.07 103.80 1.76 99.50 2.36 -0.51 4.39
1.84 93.30 2.43 98.20 1.10 12.30 2.82
16.60 99.80 2.86 89.60 1.00 -7.28 3.29
Formononetin 0.07 100.10 1.62 100.30 3.21 15.50 3.76
1.84 93.20 1.91 99.70 1.25 8.90 3.26
16.60 99.70 2.68 90.50 0.95 -7.12 3.39
  1. aExtraction efficiency (%) = 100% × amount found (pre-extraction) /amount found (after extraction). The data was presented as average of 6 independent determinations, and the SD was < 5% of the Mean, which was not shown for clarity
  2. bMatrix effects (%) = 100% × amount found (after extraction)/amount spiked.The data was presented as average of six independent determinations, and the SD was < 5% of the Mean, which was not shown for clarity
  3. cAutosampler stability (%) = 100% × amount found (pre extraction) − amount spiked/amount spiked. The data was presented as average of 6 independent determinations, and the SD was < 5% of the Mean, which was not shown for clarity
  4. Cal-O-β-glu calycosin-7-O-β-D-glucoside