Fig. 1

Micronized sacchachitin (mSC) in concentration of 200ug/mL significantly enhanced the proliferation of Pax7 + ve SCs isolated from TA muscle. a Co-immunostaining using anti-Pax7 antibody and BrdU assay was performed 2 days after cell plating. Pax7 single positive (Pax7 + ve, white arrow), BrdU single positive (BrdU + ve, yellow arrow) and Pax7-BrdU double positive (Pax7 + ve BrdU + ve, white arrow head) were counted within each group. Scale bar is 30 μm. b Micronized sacchachitin (mSC) of 200 μg/ml significantly enhanced the proliferation of Pax7 + ve cells (relative to control, 1.160 ± 0.96, *p < 0.05, n = 6, one-way ANOVA with Dunnett’s multiple comparisons). c 8 days after cell plating, MyHC protein within mediums contained different mSC concentration was evaluated through Western blotting assay. Compatible with the results of Pax7 + ve BrdU + ve cell number, mSC of 200 μg/ml showed the highest MyHC (Myosin Heavy Chain) protein expression (0.459 ± 0.068, *p < 0.05, one-way ANOVA with Dunnett’s multiple comparisons)