Fig. 3

Trilobatin regulated the phosphorylation of IRS1 at Ser612 (a), Ser307 (b) and AKT at Thr 308 (c), Ser 473 (d) in palmitate-induced C2C12 myotubes. After C2C12 myotubes were incubated with 0.2 mM palmitate (PA) without or with indicated doses of trilobatin (Tri) for 24 h, the cells were washed once and stimulated with 100 nM insulin for 15 min. The cell lysates were used to determine the expression and phosphorylation of IRS1 at Ser 612 (a), Ser 307 (b) and AKT at Thr 308 (c), Ser 473 (d). Data are mean ± SD from three independent experiments. ^@@p < 0.01 vs. control (no insulin stimulation), **p < 0.01 vs. 100 nM insulin alone, ^##p < 0.01 vs. 0.2 mM PA plus 100 nM insulin stimulation