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Fig. 5 | Chinese Medicine

Fig. 5

From: Trilobatin ameliorates insulin resistance through IRS-AKT-GLUT4 signaling pathway in C2C12 myotubes and ob/ob mice

Fig. 5

LY294002, an PI3K inhibitor, prevented the effects of trilobatin on glucose uptake and GLUT4 distribution in insulin-resistant C2C12 myotubes. After C2C12 myotubes were incubated with 0.2 mM palmitate (PA) without or with indicated doses of trilobatin (Tri) for 24 h, the cells were washed once and stimulated with 100 nM insulin for 30 min in the presence or absence of 10 μM LY294002 (LY), and then incubated with 2-NBDG for 30 min. a The uptake of 2-NBDG was determined as described in “Methods”. b, c the protein of GLUT4 in membrane and cytoplasm was detected with western blot. d The ratio of GLUT4 in membrane and cytoplasm was calculated. Data are mean ± SD from three independent experiments. @@p < 0.01 vs. control (no insulin stimulation), **p < 0.01 vs. 100 nM insulin alone, ##p < 0.01 vs. 0.2 mM PA with 100 nM insulin stimulation, $p < 0.05, $$p < 0.01 vs. 0.2 mM PA combined with 10 μM Trilobatin and 100 nM insulin

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