Fig. 8

Trilobatin regulated the phosphorylation of IRS1 and AKT in muscle tissues of ob/ob mice. After the ob/ob mice were administrated with 10 mg/kg trilobatin (i. g.) for 4 weeks, the skeletal muscles were collected, and proteins were extracted, the expression of IRS1 and AKT, the phosphorylation of IRS1 at Ser 612 (a) and Ser 307 (b), and the phosphorylation AKT at Thr 308 (c) and Ser 473 (d) were detected with western blotting assay. Data are mean ± SD of randomly selected three from ten mice, and the experiments were repeated at least three times. **p < 0.01 vs. C57BL6, ^##p < 0.01 vs. ob/ob