Fig. 2

Effects of BCB on ROS generation and its ovotoxicity in CHO-K1 cells. a Cytotoxicity of BCB in CHO-K1 cells. Cells were treated with various concentrations of BCB extract (10, 50, 100, 300, or 500 µg/mL) for 24 h. Cell viabilities were measured using an MTT assay. Results are presented as percentages of vehicle-treated controls. b Effect of BCB extract on ROS generation. CHO-K1 cells were treated with 100 μM H₂O₂ with or without 100 μg/mL BCB. Fold increases in ROS versus vehicle-treated controls, were determined by measuring DCF fluorescence intensities. c Effect of BCB extract on ovotoxicity. CHO-K1 cells were pretreated with different concentrations (50–500 μg/mL) of BCB for 2 h and then treated with 1.5 mM VCD for 24 h. (significant vs. vehicle-treated controls, **p < 0.01; significant vs. VCD or H₂O₂ treated cells, ^##p < 0.01)