Fig. 2

Apoptosis of hepatocytes induced by H/R injury was inhibited by GBDP pretreatment. a Apoptotic cells were determined by Annexin V staining in the AML-12 cells. Cells were pretreated with GBDP (60 or 120 µg/mL) for 1 h followed by incubation in the hypoxia chamber for 12 h and reoxygenation for 22 h. b Apoptotic rate of AML-12 cells was shown on the right (n = 3 per group). The protein expression of apoptosis-related markers was detected by western blot analysis. c AML-12 cells were pretreated with GBDP (60 or 120 µg/mL) for 1 h. The protein samples were collected after 12 h hypoxia and 24 h reoxygenation. The expression of Bax, Bcl-2, PARP-1, and caspase-3 was detected. d The quantification results were shown as below (n = 3 per group). e Primary hepatocytes were isolated from at least 3 different C57BL/6 mice, and pretreated with GBDP for 1 h. After 6 h hypoxia and 3 h reoxygenation, protein samples were obtained. The protein expression of apoptosis-related markers was determined. f The quantification results were shown as below (n = 3 per group). *p < 0.05, **p < 0.01, and ***p < 0.001 vs Control group; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 vs H/R group