Fig. 3

GBDP pretreatment attenuated the liver I/R injury in mice. a Flow diagram of the in vivo experiments. The samples of blood and liver tissue were collected, and the following analyses were performed: ALT, AST, and ELISA assay (6 h of reperfusion), western blotting for apoptotic proteins and histology (24 h of reperfusion). b The plasma levels of ALT and c AST were analyzed after 6 h of reperfusion. Values were expressed as mean ± standard error of the mean (n = 6 per group). d After 24 h of reperfusion, liver necrosis was assessed by H&E staining. Bars=100 μm.***p<0.001 vs Sham group;^##p<0.01 and ^###p<0.001 vs I/R group