Fig. 1

Anemoside B4 ameliorates TNBS-induced colitis in rats. Rats were orally administrated with 80 mg/kg TNBS for one day and then were continuously injected with anemosdie B4 at the doses of 5 and 10 mg/kg twice per day for 7 days. Mesalazine was administrated consecutively i.g. at the dose of 200 mg/kg once per day for 7 days. Rats were sacrificed on the 8th day after colitis induction. a Body weight of each group was recorded during the progression. b DAI of each group during the disease process. c Colon length of rats from different groups. d Representative images of colons at the end of the experiment. Scale bar, 100 µm. Results in (a–c) are presented as mean ± SD (n = 7). Body weight change and disease activity index, two-way ANOVA followed by Dunnett’s multiple comparisons test. ^###: P < 0.001, vs. vehicle group, *: P < 0.05, **: P < 0.01, ***: P < 0.001 vs. TNBS + saline group