Fig. 5
From: Anemoside B4 ameliorates TNBS-induced colitis through S100A9/MAPK/NF-κB signaling pathway
Anemoside B4 attenuates the effect of S100A9 recombinant protein on the phosphorylation and activation of NF-κB and MAPKs in Caco-2 cells. Human Caco-2 cells were treated with S100A9 recombinant protein (1 µg/mL) for 1 h, and cell were treated with anemoside B4 for 6 h. a Phosphorylation of p65, ERK1/2, JNK1/2 and p38 MAPK and total proteins were analyzed by Western blotting. b Analysis of S100A9 and its downstream protein level. Data were shown as mean ± SD (n = 3). Each experiment was repeated for three times. #: P < 0.05 vs. control group. *: P < 0.05 vs. S100A9 group