Fig. 2From: 13-Methylberberine improves endothelial dysfunction by inhibiting NLRP3 inflammasome activation via autophagy induction in human umbilical vein endothelial cells13-MB attenuates H2O2-induced apoptosis in HUVECs. HUVECs were pretreated with 1 µM 13-MB for 24 h prior to 100 µM H2O2 for 6 h. Then the cells were incubated with Annexin V-FITC/PI double staining. The rate of apoptosis was evaluated by flow cytometry. ****P < 0.001Back to article page