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Fig. 6 | Chinese Medicine

Fig. 6

From: 13-Methylberberine improves endothelial dysfunction by inhibiting NLRP3 inflammasome activation via autophagy induction in human umbilical vein endothelial cells

Fig. 6

13-MB preserves mitochondrial membrane potential via autophagy induction in HUVECs. HUVECs were treated with 13-MB prior to H2O2, with the pretreatment of SAR405(1 μM) for 1 h, or pretreatment of BAF (100 nM) for 1 h. After that, the cells were incubated with JC-10 at 5 µM for 30 min in the dark. The fluorescent intensity for both aggregate and monomeric forms of JC-10 were measured with fluorescence microscopy. Red/green fluorescence ratios per cell were calculated. Scale bar = 100 µm. *P < 0.05, **P < 0.01, ***P < 0.001

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