Fig. 5From: Shikonin promotes ubiquitination and degradation of cIAP1/2-mediated apoptosis and necrosis in triple negative breast cancer cellsSKO promoted ubiquitination and proteasome degradation of cIAP1/cIAP2 and interfered RIP1 prosurvival signaling. a The relative viability of MDA-MB-231 cells treated with or without 5 µM LAC and 2.5 µM SKO. b The ubiquitination of proteins in cells treated with or without 5 µM LAC and 2.5 µM SKO. c Immunoprecipitation assay for detecting ubiquitination of cIAP2 and interaction with RIP1 in cells treated with or without 5 µM LAC and 2.5 µM SKO. d Immunoprecipitation assay for detecting ubiquitination of cIAP1 and interaction with RIP1 in cells treated with or without 5 µM LAC and 2.5 µM SKO. e The relative viability of cells treated with or without 10 µM Nec-1 and 2.5 µM SKO. Data was represented as means ± SEM. **p < 0.01 vs. untreated control group. #p < 0.01 compared with the corresponding groupBack to article page