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Table 1 The chromatographic and mass spectrometric data of galli gigeriae endothelium corneum ethyl acetate extract using HPLC-QTOF–MS/MS

From: Galli gigeriae endothelium corneum: its intestinal barrier protective activity in vitro and chemical composition

  RT Precusor ion (amu)M Selective ion m/z Mass error (ppm) Formula Observed diagnostic CID fragment ions Identification
Measured Calculated
1 2.28 113.0589 [M + H]+ 114.0664 114.0662 − 1.87 C4H7N3O 72.0562 Creatinine [19]
2 2.42 135.0538 [M + H]+ 136.0611 136.0604 − 3.45 C5H5N5 119.0342, 92.0241, 65.0132 Adenine [19]
3 2.52 151.0494 [M + H]+ 152.0559 152.0567 1.78 C5H5N5O 135.0291, 110.0347, 80.0259 Guanine [20]
4 2.77 136.0377 [M + H]+ 137.0450 137.0458 2.79 C5H4N4O 119.0345, 110.0354, 94.0384 Hypoxanthine [20]
5 3.77 267.0952 [M + H]+ 268.1025 268.1040 3.73 C10H13N5O4 136.0618 Adenosine [20]
6 3.92 122.048 [M + H]+ 123.057 123.0553 − 1.02 C6H6N2O 109.0464, 80.0465, 53.0366 Niacinamide [19]
7 4.96 168.0896 [M + H]+ 169.0969 169.0972 − 0.27 C8H12N2O2 98.0600, 70.0656 Pyridoxamine [19]
8 5.18 131.0946 [M + H]+ 132.1014 132.1019 1.85 C6H13NO2 86.0965, 69.0701, 57.0580, 44.0500 Leucine/isoleucine [21]
9 5.97 129.0574 [M + H]+ 130.0647 130.0651 3.3 C9H7N 103.0546, 77.0385 Isoquinoline [20]
10 11.16 173.1052 [M + H]+ 174.1146 174.1125 − 2.31 C8H15NO3 90.0492, 57.0671 Acly-glycine [21]
11 12.00 416.1075 [M + H]+ 417.1148 417.1180 0.71 C21H20O9 255.0627 Daidzin [23]
12 12.36 446.1176 [M + H]+ 447.1249 447.1286 2.3 C22H22O10 285.0751 Glycitin [23]
13 12.37 446.0817 [M + H]+ 447.0890 447.0902 3.3 C21H18O11 271.0591 Genistein-O-glucuronide [24]
14 13.02 276.1090 [M + H]+ 277.1163 277.1183 0.3 C14H16N2O4 231.1117, 186.0894 N-lactoyl-tryptophan [21]
15 13.50 210.1348 [M + H]+ 211.1421 211.1441 2.4 C11H18N2O2 138.1272, 70.0655 L,L-Cyclo(leucylprolyl) [22]
16 15.03 502.1074 [M + H]+ 503.1147 503.1184 0.01 C24H22O12 255.0631 6″-O-Malonyldaidzin [24]
17 15.20 432.103 [M + H]+ 433.1103 433.1129 2.68 C21H20O10 271.0581 Genistin [23]
18 15.74 175.0621 [M + H]+ 176.0730 176.0706 − 1.11 C10H9NO2 130.0588 Indoleacetic acid [30]
19 17.02 458.1188 [M + H]+ 459.1261 459.1286 2.34 C23H22O10 255.0634 6″-O-Acetyldaidzin [24]
20 17.28 488.1288 [M + H]+ 489.1361 489.1391 3.15 C24H24O11 285.0737 6″-O-Acetylglycitin [24]
21 17.91 518.1029 [M + H]+ 519.1102 519.1133 − 0.55 C24H22O13 271.0584 6″-Malnoylgenistin [24]
22 18.67 145.0527 [M + H]+ 146.0602 146.0621 0.28 C9H7NO 91.0547, 77.0392 1H-Indole-3-carboxaldehyde [31]
23 20.35 474.1146 [M + H]+ 475.1219 475.1235 3.35 C23H22O11 271.0578 6″-O-Acetylgenistin [24]
24 21.25 254.0563 [M + H]+ 255.0636 255.0652 3.24 C15H10O4 237.0527, 137.0223 Daidzein [23]
25 21.76 284.0666 [M + H]+ 285.0739 285.0757 2.51 C16H12O5 270.0501, 242.0548 Glycitein [23]
26 25.21 515.2909 [M + H] 514.2836 514.2844 1.55 C26H45NO7S 353.2490, 124.0074, 106.9810, 80.0027 Taurocholic acid [28, 29]
27 26.15 465.3094 [M + H] 464.3021 464.3018 − 0.73 C26H43NO6 435.3073, 402.2974, 74.0251 Glycocholic acid [27]
28 26.72 270.0511 [M + H]+ 271.0584 271.0601 3.29 C15H10O5 153.0171 Genistein [23]
29 28.14 406.2692 [M + H] 405.2619 405.2636 3.76 C24H38O5 389.2649, 371.2541, 353.2437 Oxocholic acid [26]
30 30.31 499.2969 [M + H] 498.2896 498.2895 − 0.24 C26H45NO6S 480.2758, 124.0074, 106.9810, 80.0027 Taurochenodeoxycholic acid [28]
31 31.23 408.2861 [M + H] 407.2803 407.2788 3.67 C24H40O5 389.2641, 343.2629, 289.2168 Cholic acid [25, 26]
32 32.09 449.3138 [M + H] 448.3065 448.3068 0.77 C26H43NO5 405.2652, 386.3051, 330.2422, 74.0251 Glycochenodeoxycholic acid [27]
34 39.88 392.2925 [M + H] 391.2852 391.2854 0.47 C24H40O4 373.2694 Chenodeoxycholic acid [25, 26]