Fig. 4

Protein expression level of MAPKs pathway. Protein were extracted from livers and western blotting was used to analysis protein expression level. The lysate sample of each group was a mixture of 6 mice from the same group (n = 6 per group). The mixture sample of each group was prepared 3 times and the experiments were repeated at least 3 times. β-actin was used as a loading control a p-ERK and ERK protein expression level. b Quantity of p-ERK protein relative expression to ERK. c p-p54/p-p46 SAPK/JNK and SAPK/JNK protein expression. d Quantity of p-p54/p-p46 SAPK/JNK relative protein expression to SAPK/JNK. e p-p38 and p38 protein expression. f Quantity of p-p38 relative protein expression to p38. Data are expressed as mean ± SD. P value < 0.05 were considered significant.*P < 0.05 vs MCD diet, **P < 0.05 vs MCD diet, ***P < 0.05 vs MCD diet, #P < 0.05 vs MCS diet, ##P < 0.01 vs MCS diet