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Fig. 5 | Chinese Medicine

Fig. 5

From: Hepatoprotective effect of Qushihuayu formula on non-alcoholic steatohepatitis induced by MCD diet in rat

Fig. 5

The potential mechanism of QSHY formula on inflammation and HSCs reprogramming. Protein and RNA were extracted from livers, western blotting and real-time PCR were used to analysis protein and mRNA expression. Nucleus protein extraction kit was used to analysis protein expression level in nucleus. The lysate sample of each group was a mixture of 6 mice from the same group (n = 6 per group). The mixture sample of each group was prepared 3 times and the experiments were repeated at least 3 times. β-actin was used as a loading control a PPRA-γ protein expression in nucleus, Lamin B1 protein expression, PPRA-γ protein expression in whole cell and β-actin protein expression. b Quantity of PPRA-γ protein relative expression in nucleus to Lambin B1. c Quantity of PPRA-γ protein relative expression in whole cell to β-actin. d PPRA-γ relative mRNA expression. e p-p65 and Lamin B1 protein expression in nucleus. f Quantity of p-p65 relative protein expression to Lamin B1. g HNF4A relative mRNA expression. h FOXA3 relative mRNA expression. i HNF1A relative mRNA expression Data are expressed as mean ± SD. P value < 0.05 were considered significant.*P < 0.05 vs MCD diet, **P < 0.05 vs MCD diet,***P < 0.05 vs MCD diet, #P < 0.05 vs MCS diet, ##P < 0.01 vs MCS diet, ###P < 0.001 vs MCS diet

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