No | Analyte | RT (min) | Transition | Cone voltage (V) | Collision energy (V) | Calibration curve | LOD (ng/mL) | LOQ (ng/mL) | Precision (RSD, %) | Recovery (%, n = 6) | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Equation | R2 | Range (μg/mL) | Intra-day | Inter-day | |||||||||
1 | Geniposidic acid | 2.05 | 375.40 → 195.24 | 15 | + 15 | y = 260.2x − 43.8 | 0.9985 | 0.064–16.46 | 8.04 | 32.1 | 4.44 | 5.74 | 94.2 |
2 | Protocatechuic acid | 2.26 | 155.20 → 65.13 | 30 | + 20 | y = 1781.6x + 43.5 | 0.9995 | 0.009–9.340 | 0.65 | 2.28 | 2.59 | 5.15 | 97.8 |
3 | Chlorogenic acid | 3.35 | 355.30 → 163.08 | 20 | + 15 | y = 19714x − 689 | 0.9973 | 0.007–3.575 | 0.10 | 0.33 | 4.89 | 5.80 | 90.8 |
4 | Scopolin | 3.47 | 355.11 → 193.05 | 25 | + 15 | y = 20562x − 1022 | 0.9987 | 0.005–5.550 | 0.18 | 0.60 | 2.91 | 3.04 | 104.2 |
5 | Puerarin | 4.03 | 417.30 → 297.20 | 46 | + 25 | y = 10069x + 1933 | 0.9954 | 0.007–13.89 | 0.03 | 0.11 | 2.60 | 3.13 | 101.2 |
6 | Magnoflorine | 4.45 | 342.30 → 297.30 | 42 | + 19 | y = 27481x + 1247 | 0.9992 | 0.001–5.542 | 0.25 | 0.74 | 1.87 | 3.99 | 104.8 |
7 | Purpureaside C | 4.64 | 787.46 → 163.03 | 20 | + 30 | y = 444.3x + 34.7 | 0.9992 | 0.022–11.15 | 5.44 | 21.7 | 1.06 | 3.70 | 103.1 |
8 | Daidzin | 4.76 | 417.36 → 255.22 | 30 | + 20 | y = 17278x + 4508 | 0.9991 | 0.009–36.47 | 0.04 | 0.14 | 1.18 | 3.38 | 98.0 |
9 | Echinacoside | 4.91 | 787.55 → 325.10 | 20 | + 20 | y = 317.6x + 5.9 | 0.9995 | 0.046–11.78 | 2.16 | 7.66 | 3.38 | 3.51 | 90.9 |
10 | Rutinum | 5.63 | 611.20 → 303.10 | 35 | + 15 | y = 12785x + 160 | 0.9991 | 0.003–2.600 | 0.05 | 0.15 | 3.17 | 5.20 | 95.6 |
11 | Calycosin-7-glucoside | 5.70 | 447.28 → 285.19 | 36 | + 17 | y = 47510x + 2889 | 0.9962 | 0.001–5.725 | 0.02 | 0.05 | 2.08 | 2.41 | 107.6 |
12 | Ferulic acid | 5.74 | 195.10 → 117.00 | 20 | + 23 | y = 10691x + 201 | 0.9992 | 0.004–4.250 | 0.06 | 0.24 | 4.42 | 5.16 | 100.6 |
13 | Hyperoside | 5.79 | 465.27 → 303.16 | 26 | + 15 | y = 14954x − 199 | 0.9994 | 0.007–3.650 | 0.09 | 0.34 | 2.82 | 2.85 | 97.1 |
14 | Crustecdysone | 6.04 | 481.41 → 371.34 | 30 | + 15 | y = 881.7x + 54.6 | 0.9999 | 0.017–16.91 | 4.13 | 16.5 | 2.44 | 3.07 | 99.7 |
15 | Acteoside | 6.07 | 625.39 → 163.12 | 20 | + 30 | y = 693.3x + 52.6 | 0.9989 | 0.015–7.721 | 3.77 | 11.3 | 4.50 | 5.72 | 99.4 |
16 | Coumarin | 7.41 | 147.25 → 91.16 | 30 | + 30 | y = 20078x + 89 | 0.9999 | 0.001–4.853 | 0.04 | 0.19 | 3.16 | 3.65 | 103.4 |
17 | Ononin | 8.10 | 431.26 → 269.26 | 30 | + 25 | y = 31798x + 1102 | 0.9997 | 0.002–7.794 | 0.03 | 0.10 | 1.47 | 3.36 | 103.9 |
18 | Daidzein | 8.19 | 255.23 → 199.41 | 30 | + 25 | y = 12439x + 90 | 0.9998 | 0.001–1.131 | 0.07 | 0.28 | 0.68 | 3.05 | 96.3 |
19 | Salvianolic acid A | 8.51 | 495.17 → 223.19 | 20 | + 40 | y = 984.7x − 53.3 | 0.9980 | 0.017–8.906 | 4.35 | 11.6 | 0.72 | 1.79 | 106.9 |
20 | Quercetin | 8.92 | 303.60 → 229.05 | 50 | + 30 | y = 3438x + 205 | 0.9990 | 0.013–13.00 | 0.71 | 2.39 | 3.02 | 3.45 | 91.9 |
21 | Epimedin A1 | 8.98 | 839.58 → 369.27 | 36 | + 34 | y = 9091x + 223 | 0.9996 | 0.007–6.700 | 0.68 | 2.18 | 4.40 | 4.73 | 104.5 |
22 | Epimedin B | 9.31 | 809.57 → 369.22 | 30 | + 34 | y = 7352x + 1340 | 0.9924 | 0.007–13.80 | 0.19 | 0.75 | 1.08 | 3.85 | 99.4 |
23 | Epimedin C | 9.48 | 823.58 → 369.27 | 34 | + 35 | y = 8289x + 32 | 0.9998 | 0.005–10.25 | 0.71 | 2.11 | 1.48 | 1.90 | 94.8 |
24 | Icariin | 9.66 | 677.48 → 369.22 | 38 | + 27 | y = 12010x + 1110 | 0.9987 | 0.002–12.44 | 0.01 | 0.03 | 3.23 | 4.01 | 92.2 |
25 | Formononetin | 11.88 | 269.19 → 253.11 | 40 | + 30 | y = 10680x + 39 | 0.9997 | 0.001–1.011 | 0.02 | 0.06 | 1.82 | 2.44 | 111.7 |
26 | Baohuoside II | 12.03 | 501.29 → 355.24 | 30 | + 15 | y = 20952x − 126 | 0.9997 | 0.002–3.456 | 0.03 | 0.11 | 1.47 | 5.18 | 106.5 |
27 | Eugenol | 12.13 | 164.07 → 103.13 | 44 | + 21 | y = 185.8x + 134.1 | 0.9986 | 0.035–35.52 | 8.54 | 27.7 | 3.63 | 3.72 | 90.3 |
28 | Astragaloside A | 12.37 | 785.50 → 455.40 | 25 | + 15 | y = 947.3x + 6.2 | 0.9996 | 0.012–3.028 | 0.80 | 2.00 | 3.15 | 4.29 | 99.3 |
29 | Rhamnocitrin | 12.39 | 301.10 → 258.10 | 50 | + 30 | y = 17437x − 344 | 0.9989 | 0.003–2.900 | 0.06 | 0.22 | 4.54 | 5.38 | 106.4 |
30 | Chikusetsusaponin iva | 12.74 | 793.57 → 793.57 | 30 | -20 | y = 1110x + 21 | 1.0000 | 0.010–9.779 | 0.30 | 1.19 | 3.60 | 4.10 | 92.3 |
31 | Geniposide | 13.01 | 389.43 → 255.29 | 30 | + 25 | y = 72.6x + 4.7 | 0.9998 | 0.092–11.84 | 23.1 | 46.2 | 3.64 | 5.15 | 102.0 |
32 | Sagittatoside A | 13.16 | 677.33 → 369.24 | 30 | + 15 | y = 6603x + 21 | 0.9999 | 0.003–2.978 | 0.73 | 2.91 | 2.53 | 4.49 | 95.8 |
33 | Astragaloside II | 13.31 | 827.75 → 175.09 | 20 | + 30 | y = 579.6x + 9.0 | 0.9992 | 0.004–2.243 | 0.73 | 2.19 | 3.49 | 5.81 | 106.2 |
34 | Sagittatoside B | 13.45 | 647.45 → 369.22 | 24 | + 24 | y = 9750x + 95 | 0.9998 | 0.004–3.600 | 0.59 | 2.34 | 4.79 | 4.88 | 109.1 |
35 | Icariside I | 13.48 | 531.19 → 369.14 | 35 | + 30 | y = 26745x + 119 | 0.9991 | 0.000–0.563 | 0.03 | 0.07 | 2.70 | 3.96 | 97.5 |
36 | 2′'-O-rhamnosyl Icariside II | 13.51 | 661.47 → 369.28 | 28 | + 21 | y = 14713x + 522 | 0.9979 | 0.003–5.150 | 0.11 | 0.38 | 3.47 | 4.15 | 90.6 |
37 | Baohuoside I | 13.83 | 515.30 → 369.20 | 26 | + 20 | y = 48629x + 525 | 0.9988 | 0.000–3.375 | 0.03 | 0.10 | 0.99 | 2.69 | 96.8 |
38 | Desmethyl Icaritin | 13.87 | 355.10 → 299.10 | 50 | + 20 | y = 129383x + 2666 | 0.9991 | 0.001–3.950 | 0.03 | 0.08 | 2.37 | 3.43 | 95.4 |
39 | Astragaloside I | 14.11 | 869.74 → 217.27 | 15 | + 15 | y = 854.4x + 32.2 | 0.9994 | 0.009–8.750 | 1.07 | 4.27 | 1.02 | 1.99 | 101.4 |
40 | Z-ligustilide | 14.73 | 191.11 → 117.01 | 38 | + 22 | y = 8945x − 92 | 0.9998 | 0.005–11.00 | 0.86 | 2.94 | 4.27 | 4.68 | 98.8 |
41 | Icaritin | 15.38 | 369.14 → 313.07 | 50 | + 26 | y = 220062x + 3559 | 0.9959 | 0.000–0.322 | 0.00 | 0.01 | 3.13 | 4.85 | 106.9 |
42 | Tanshinone IIA | 16.04 | 295.29 → 277.24 | 50 | + 19 | y = 64914x + 4212 | 0.9911 | 0.001–3.200 | 0.05 | 0.17 | 2.06 | 2.68 | 104.9 |
43 | Oleanolic acid | 16.69 | 439.36 → 203.18 | 35 | + 25 | y = 19247x + 370 | 0.9981 | 0.002–1.611 | 0.21 | 0.53 | 3.08 | 3.60 | 92.3 |