Skip to main content
Fig. 2 | Chinese Medicine

Fig. 2

From: Aqueous extracts of Aconite promote thermogenesis in rats with hypothermia via regulating gut microbiota and bile acid metabolism

Fig. 2

AA promoted browning of WAT and thermogenesis of BAT. a White adipose tissue (WAT) weight. H&E staining of WAT (Magnification = 100×; scale bar, 100 μm) (b) and distribution of adipocyte size from WAT (c). Significance was represented by *P < 0.05 vs. control group and #P < 0.05 vs. CH group. Immunohistochemistry of uncoupling protein 1 (UCP1) of WAT (Magnification = 100×; scale bar, 100 μm) (d) and the expression of UCP1 in WAT (e). f Brown adipose tissue (BAT) weight. g H&E staining of BAT (Magnification = 200×; scale bar, 50 μm). (h and i) Immunohistochemical staining of UCP1 in BAT (Magnification = 200×; scale bar, 50 μm) (h) and the relative expression of UCP1 in BAT (i). Control, CH, and CH + AA represent groups intermittently exposed to warm water (32 ± 0.5 °C), cold water (3.5 ± 0.5 °C), and cold water plus AA, respectively. Data were expressed as mean ± standard deviation (SD) (n = 6 rats/group). Differences were evaluated by one-way ANOVA with a post hoc Student–Newman–Keuls test (*P < 0.05, **P < 0.01, ***P < 0.001, and ns represents no significant difference)

Back to article page