Fig. 1

Effects of PP7 on cell viability, motility, invasion, and endothelial tube formation in vitro. a HepG2 and HUVECs cells were treated with indicated concentrations of PP7 for 24 h and the cell viability was assessed using an MTT assay. b HepG2 and HUVECs cells were scratched with a pipette tip, and the cells were exposed to different concentrations of PP7 for 24 h. c Quantification of relative distance of the denuded zone of b. d HepG2 and HUVECs cells were incubated with various concentrations of PP7 for 24 h. Representative images are shown. e Quantification of migrating and invasive cells of d. f HUVEC cells were plated on Matrigel and treated with increasing concentrations of PP7 for 24 h. Capillary tube formation was observed and photographed at × 200. g Quantification of branch points of f. Values are expressed as means ± SD of triplicate experiments. *P < 0.05, **P < 0.01, versus control groups